Procoagulant Activity on Platelets Adhered to Collagen or Plasma Clot

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Procoagulant activity on platelets adhered to collagen or plasma clot.

In a new 2-stage assay of platelet procoagulant activity (PCA), we first subjected gel-filtered platelets to adhesion on collagen (as a model of primary hemostasis) or plasma clots (as a model of preformed thrombus) for 30 minutes, and then the adherent platelets were supplemented with pooled, reptilase-treated, diluted plasma. Defibrinated plasma provided coagulation factors for assembly on pl...

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Procoagulant Activity of Adherent Platelets

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Procoagulant Activity of Adherent Platelets

The possibility that platelets release microvesicles on adherence to either von Willebrand factor (vWf) or collagen was examined by flow cytometry analysis of the supernatant above layers of adherent platelets. No microvesicle release was detected as a result of adherence to vWf or to collagen, a known platelet agonist. Approximately 8% of the total platelet mass was released as microvesicles a...

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Procoagulant Platelets

Procoagulant platelets are known by several synonyms, including collagen and thrombin–activated platelets, coated platelets, sustained calcium–induced platelet morphology, superactivated platelets, and superplatelets. This assortment of names is confusing to those outside the field and has remained a stubborn problem within the field. However, it is key to recognize that all of these variously ...

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The effect of fibrin clots and clot-bound thrombin on the development of platelet procoagulant activity.

We tested different types of clot for their ability to provoke procoagulant activity in platelets: normal clots from platelet poor plasma (des AABB- or fibrin II clots), similar clots in which the adsorbed thrombin has been inhibited by hirudin, and clots obtained by the action of two snake venom enzymes that release only fibrinopeptide A (des AA- or fibrin I clots). Analogous clots from fibrin...

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ژورنال

عنوان ژورنال: Arteriosclerosis, Thrombosis, and Vascular Biology

سال: 2001

ISSN: 1079-5642,1524-4636

DOI: 10.1161/01.atv.21.4.628